dy 268  (Tocris)

Journal: Cell Death Discovery

Article Title: The bacterial metabolite, lithocholic acid, has antineoplastic effects in pancreatic adenocarcinoma

doi: 10.1038/s41420-024-02023-1

Figure Lengend Snippet: Capan-2 cells were treated with DMSO or LCA (0.03 µM) and/or 5 µM CINPA1, DY268, NF449, GSK2033, and ketoconazole for 48 h. ( A ) Cell invasion was measured using Corning Matrigel invasion chambers. B β-catenin and NRF2 protein levels were measured by western blotting (representative figure). TGR5, FXR, and VDR bile acid receptors were transiently silenced in Capan-2 cells using the corresponding siRNA. A control group was transfected with negative control siRNA. After 48 h, C the transfection efficiency was assessed by qPCR, and D β-catenin expression was assessed by western blotting ( n = 3). Data are presented as means ± SEM. Transfection efficiencies were compared with one-way ANOVA and Dunnett’s post hoc. Control siRNA vs. LCA/siRNA treated groups were compared with paired t -tests. *, **, and *** indicate p < 0.05, p < 0.01, and p < 0.001. DMSO dimethyl sulfoxide, FXR farnesoid X-activated receptor, LCA lithocholic acid, NRF2 nuclear factor, erythroid 2-like 2, TGR5 Takeda G-protein coupled receptor,VDR vitamin D receptor.

Article Snippet: BA receptor antagonists (NF449 [ ], CINPA1 [ ], DY268 [ ], and GSK2033 [ ]) were acquired from Tocris Bioscience (Bristol, UK), and ketoconazole [ ] was purchased from Sigma-Aldrich.

Techniques: Western Blot, Control, Transfection, Negative Control, Expressing